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RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp <t>DNA</t> ladder (NEXmark GenesLab, Korea); lanes <t>1–2,</t> <t>AgNP</t> 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.
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RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp <t>DNA</t> ladder (NEXmark GenesLab, Korea); lanes <t>1–2,</t> <t>AgNP</t> 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.
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RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp <t>DNA</t> ladder (NEXmark GenesLab, Korea); lanes <t>1–2,</t> <t>AgNP</t> 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.
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RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp <t>DNA</t> ladder (NEXmark GenesLab, Korea); lanes <t>1–2,</t> <t>AgNP</t> 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.
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RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp <t>DNA</t> ladder (NEXmark GenesLab, Korea); lanes <t>1–2,</t> <t>AgNP</t> 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.
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RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp DNA ladder (NEXmark GenesLab, Korea); lanes 1–2, AgNP 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.

Journal: Biotechnology Reports

Article Title: Silver nanoparticles enhance organogenesis, total flavonoid and anthocyanin contents in Gynura pseudochina tissue culture

doi: 10.1016/j.btre.2026.e00964

Figure Lengend Snippet: RAPD–PCR profiles of Gynura pseudochina plantlets exposed to different concentrations of silver nanoparticles (AgNPs) after 49 days of in vitro culture. (a) Amplification patterns generated using primer OPJ-20, revealing six monomorphic loci within the 550–1000 bp range across all treatments. (b) Amplification patterns generated using primer R8, showing four monomorphic loci between 400 and 1000 bp with no polymorphic bands detected. Lane M, 100 bp DNA ladder (NEXmark GenesLab, Korea); lanes 1–2, AgNP 0 ; lanes 3–4, AgNP30; lanes 5–6, AgNP60; lanes 7–8, AgNP90.

Article Snippet: Genomic DNA was extracted from G. pseudochina plantlets after 49 days of AgNP elicitation using a Genomic DNA Mini Kit (Plant) (Geneaid Biotech Ltd., Taiwan), following the manufacturer’s instructions.

Techniques: In Vitro, Amplification, Generated